Supiana Dian Nurtjahyani't dan Retno
Handajani
Fakultas Keguruan dan Ilmu Pendidikan/ Progam
Studi Pendidikan Biologi
Universitas PGRI Ronggolawe Tuban, dan Fakultas
Kedokteran dan Institute of Tropical Disease,
Universitas Airlangga
Salmonella ryphi as the cause agent of typhoid
fever caused death of people more than 600.000
yearly. The datas showed typhoid fever is an epidemic disease spread widely in tropical and subtropical areas (Mirza et al.,2000. Thong cat a/.. 2002. Wain et
al., 2003, Mandal et al.,2004). In Indonesia
chloramphenicol has been used to treat typhoid fever was used more than fifty years. The resistency of the
antibiotics has become a serious problem
in handling infectious diseases. The analysys moleculer resistance Salmonella typhy because enchanged impermeable drug or prodsuct
Chloramphenicol Acetyltransferase (CAT) inactivation
chloramphenicol.
The objective of' the study getting nucleotide
sequences of Chloramphenicol Acetyltransferase (CAT) gen resitance to
Chloramphenicol of Salmonella ryphi. The research is a cross-sectional study with
laboratory experimental method inlaboratory UNIROW Tuban dan TDC Surabaya.
Thirty samples from Typhus Abdominalis patients
were obtained from some hospitals in Tuban. The datas were analysed using qualitative analysis.PCR results shown 96,66% Salmonella typhi DNA positives in
Typhus Abdominalis patients. It was found that 3,3% (1/30) Salmonella typhi was resistance
to Chloramphenicol and 222 nucleotides of CAT
gene was 100% homolog to Salmonella typhi Ace. No. JO 1841
published before. In conclusion, sequen nucleotide Salmonella typhi
resistance in Tuban city 100% to Salmonella ryphi Ace. No. JO 1841 published before. Further study were needed for detection using PCR method
for diagnosis and CAT gene of Salmonella typhi
with dfficulties.
Key Word:
Sequen nucleotide , Salmonella typhi resistance Chloramphenicol, patient typhus
abdominalis
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